Exprese proteinu se týká syntézy Proteinů within cells using information contained in the DNA or gene.
Proteiny are responsible for all the biochemical reactions that take place within the cell. Hence, it makes it necessary to study protein function in order to understand cellular processes.
This is currently studied based on using fluorescent Proteinů as tags. However, this does not provide real time analysis as it requires maturation of chromophore that takes time and this causes a delay in investigation of real-time expression, especially for Proteinů které jsou přechodné nebo pomíjivé povahy.
Výzkumníci ohlásili 30. července 2020 na předtiskovém serveru novou techniku, která může toto omezení překonat.
The new study describes the use a fluorescent biosensor that enables real-time detection of protein expression in vivo which has implications for understanding the spatiotemporal expression of Proteinů within the living organism. This sensor is based on a dim green fluorescent protein in which pre-existing fluorescence increases 11-fold in vivo following the specific and rapid binding of a protein tag and enables detection of protein expression within seconds in live cells.
Tento biosenzor will be useful to study biological processes in real-time where Proteinů are either transiently expressed and/or to understand the expression and interactions of Proteinů from a disease-causing organism such as bacteria or a virus with that of the host proteins.
Reference:
Eason MG., Pandelieva AT., Mayer MM., et al 2020. Geneticky kódovaný fluorescenční biosenzor pro rychlou detekci exprese proteinů. Předtisk: bioRxiv 2020.07.30.229633; DOI: https://doi.org/10.1101/2020.07.30.229633
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